Composite

Part:BBa_K2587021

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-09-17)


Plux_RBS_E_T

This part contains the lysis gene E from bacteriophage PhiX174 under the control of the Plux promoter. The Plux promoter is part of the quorum sensing system of Aliivibrio fischeri. The induction of expression of this lysis gene will occur by Plux when it is induced by the other two components of the quorum sensing system, LuxI and LuxR. This construct is supposed to reduce cell density in a population density dependent manner.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI site found at 158
    Illegal BsaI.rc site found at 116


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Categories
Parameters
None